Preface Purifying Proteins for Proteomics Companion Web Site Section Ⅰ:Preparing Proteins for Proteomic Analysis 1 Role of Separation Science in Proteomics 2 Strategies for Purifying Proteins Section Ⅱ:Chromatographic Methods for Preparing Proteins for Proteomics 3 Introduction to Chromatographic Methods for Protein and Peptide Purification 4 Multidimensional Chromatography of intact Proteins 5 High-throughput Screening of Soluble Recombinant Proteins 6 lon-exchange Chromatography 7 Size-exclusion Chromatography 8 Role of Reversed-phase High-performance Liquid Chromatography in Protein Isolation and Purification 9 Hydrophobic Interaction Chromatography 1 Affinity and Immunoaffinity Chromatography 11 Dye-Ligand Affinity Chromatography in Proteomic Research 12 Immobilized Metal-ion Affinity Chromatography 13 Protein Chromatography on Hydroxyapatite 14 Chromatofocusing Section Ⅲ:Electrophoretic Methods for Preparing Proteins for Proteomics 15 Introduction to Strategies Based on Electrophoresis for Separating Proteins 16 Separation of Proteins Using Two-dimensional Gel Electrophoresis 17 Sample Preparation for High-resolution Two-dimensional Electrophoresis by Isoelectric Fractionation in a MCE 18 An Electrophoresis Method for Purifying Proteins:The Gradiflow BF4 Instrument 19 Protein Purification Using Free-flow Electrophoresis Section Ⅳ:Analysis of Purified Proteins Prior to Structural Proteomics 2 Introduction to Methods for Characterizing Purified Proteins 21 Characterization of Proteins by Mass Spectrometry 22 Analytical Ultracentrifugation:Protein Size,Conformation,and Interactions 23 Measuring the Conformational Stability of a Protein 24 Combining Surface Plasmon Resonance with Mass Spectrometry:ldentifying Binding Partners and Characterizing Interactions 25 Analysis of Carbohydrate from Glycoproteins Appendices 1.An Overview of Protein Structure 2.Measuring the Concentration of Proteins 3.Concentrating Solutions of Proteins 4.Stabilization of Proteins for Storage 5.One-dimensional Polyacrylamide Gel Electrophoresis 6.Troubleshooting Guides 7.Cautions Index
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